Histochemical differentiation between chondroitin 4-sulfate and chondroitin 6-sulfate.
نویسندگان
چکیده
منابع مشابه
Chondroitin 4-sulfate, chondroihin 6-sulfate, and darma
malography on a column of Con A-Sapharosa (Pharmacia Fine Chemicals Inc., Piscahaway, N. J.). The CEA sample was dissolved in Con A buffer (1.0 M NaCi in 0.1 M sodium acetate buffer, pH 6.0; 1 mM in each of CaCI2, MgCi2, and MnCl2) and applied to a waler-jacketed column (22 x 1.5 cm) of Con A-Sepharose. Pool 1 material was obtained by alul ing the column at 4°with 1.5 column volumes of Con A b...
متن کاملCopolymers of chondroitin 4-sulfate and chondroitin 6-sulfate in chick embryo epiphyses and other cartilage.
Tetrasaccharides were prepared from chondroitin sulfate by means of a limited degradation with chondroitinase ABC. Tetrasaccharides containing one sulfate per disaccharide unit were isolated and were found to be of three types: a tetrasaccharide with two 6-sulfated disaccharide units, a tetrasaccharide with two 4-sulfated disaccharide units, and a tetrasaccharide with one 4-sulfated disaccharid...
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While some complementary and alternative techniques have been studied scientifically, high-quality data regarding safety, effectiveness, and mechanism of action are limited or controversial for most therapies. Whenever possible, it is recommended that practitioners be licensed by a recognized professional organization that adheres to clearly published standards. In addition, before starting a n...
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A microsomal preparation from chick embryo cartilage has been shown to catalyze the incorporation of sulfate-36S from 3’-phosphoadenosine 5’-phosphosulfate-3% into microsomal glycosaminoglycan. This enzyme preparation is the same preparation that previously has been shown to catalyze the formation of chondroitin (nonsulfated) from sugar nucleotide precursors. The microsomal sulfate acceptor app...
متن کاملBiosynthesis of Chondroitin Sulfate
The role of phospholipids in the activity of UDP-D-galactose:D-xylose galactosyltransferase (galactosyltransferase I) from embryonic chick cartilage was investigated. Phospholipase C treatment of particulate galactosyltransferase I caused inactivation of this enzyme to the extent of 60 to 70% as well as hydrolysis of 75 to 80% of the membrane phospholipids. Addition of phospholipid restored act...
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ژورنال
عنوان ژورنال: The Kurume Medical Journal
سال: 1978
ISSN: 0023-5679,1881-2090
DOI: 10.2739/kurumemedj.25.45